Identification of S-locus linked PCR fragments in rye (Secale cereale L.) by denaturing gradient gel electrophoresis
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چکیده
منابع مشابه
Identification and population dynamics of yeasts in sourdough fermentation processes by PCR-denaturing gradient gel electrophoresis.
Four sourdoughs (A to D) were produced under practical conditions, using a starter obtained from a mixture of three commercially available sourdough starters and baker's yeast. The doughs were continuously propagated until the composition of the microbiota remained stable. A fungi-specific PCR-denaturing gradient gel electrophoresis (DGGE) system was established to monitor the development of th...
متن کاملAn ALMT1 gene cluster controlling aluminum tolerance at the Alt4 locus of rye (Secale cereale L).
Aluminum toxicity is a major problem in agriculture worldwide. Among the cultivated Triticeae, rye (Secale cereale L.) is one of the most Al tolerant and represents an important potential source of Al tolerance for improvement of wheat. The Alt4 Al-tolerance locus of rye contains a cluster of genes homologous to the single-copy Al-activated malate transporter (TaALMT1) Al-tolerance gene of whea...
متن کاملThe Ph1 locus from wheat controls meiotic chromosome pairing in autotetraploid rye (Secale cereale L.).
The Ph1 locus on chromosome 5B enforces strictly bivalent pairing in polyploid wheat, but the exact mechanism of its action remains unknown. Pairing restriction involves not only wheat homoeologues and all alien introgressions but also differentiated homologues. In this study we show that chromosome 5B with its Ph1 locus also controls chromosome pairing in autotetraploid rye by apparently restr...
متن کاملTowards a whole-genome sequence for rye (Secale cereale L.).
We report on a whole-genome draft sequence of rye (Secale cereale L.). Rye is a diploid Triticeae species closely related to wheat and barley, and an important crop for food and feed in Central and Eastern Europe. Through whole-genome shotgun sequencing of the 7.9-Gbp genome of the winter rye inbred line Lo7 we obtained a de novo assembly represented by 1.29 million scaffolds covering a total l...
متن کاملDevelopment of new PCR-based markers specific for chromosome arms of rye (Secale cereale L.).
PCR-based rye (Secale cereale L.) chromosome-specific markers can contribute to the effective utilization of elite genes of rye in wheat (Triticum aestivum L.) breeding programs. In the present study, 578 new PCR-based rye-specific markers have been developed by using specific length amplified fragment sequencing (SLAF-seq) technology, and 76 markers displayed different polymorphism among rye K...
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ژورنال
عنوان ژورنال: The Plant Journal
سال: 1994
ISSN: 0960-7412,1365-313X
DOI: 10.1046/j.1365-313x.1994.5060891.x